Detection of protein in polyacrylamide gels using an improved silver stain |
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Authors: | T Marshall |
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Institution: | Chemistry Division, National Board of Occupational Safety and Health, S-171 84 Solna, Sweden |
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Abstract: | A much improved silver staining procedure for the detection of protein in polyacrylamide gels of 0.8-3.0 mm thickness is described. It achieves very high sensitivity (detecting less than 0.01 ng bovine serum albumin/mm2) by overstaining and subsequently removing nonspecific background stain using a modified, reliable destaining procedure. Maximum sensitivity follows prediamine equilibration in 0.1% (w/v) formaldehyde solution. With two-dimensional electrophoresis the improved staining procedure reveals greater than 200 polypeptides in unconcentrated human urine and greater than 150 polypeptides in a single human fingerprint. |
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Keywords: | HPLC drug metabolites HPLC techniques microsomes drug metabolism transferase metabolites |
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