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Evidence for the presence of functional beta-adrenoceptor along the proximal tubule of the rat kidney |
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| Authors: | W R Jacobs Y L Chan |
| Affiliation: | 1. Laboratory of Molecular Modeling and Design, Faculty of Pharmacy, Federal University of Goiás, Goiânia, GO 74605-220, Brazil;2. Laboratory for Molecular Modeling, Division of Chemical Biology and Medicinal Chemistry, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC 27599, USA;3. Laboratory of Theoretical Chemistry, A.V. Bogatsky Physical–Chemical Institute NAS of Ukraine, Odessa 65080, Ukraine;4. ILS/Contractor supporting the NTP Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM), P.O. Box 13501, Research Triangle Park, NC 27709, USA |
| Abstract: | Evidence for the presence of beta adrenoceptors on proximal tubules from the rat kidney has been obtained using enriched tubule suspensions prepared by Percoll centrifugation. Intact tubules demonstrated simultaneous enrichment of parathyroid hormone and isoproterenol sensitive cAMP production with no enrichment of antidiuretic hormone sensitive cAMP production. Both norepinephrine and epinephrine were less potent than isoproterenol and the stimulatory effect of catecholamines could be blocked with propranolol but not phentolamine. The stimulatory effect of norepinephrine on cellular phenylalanine uptake is blunted by co-addition of isoproterenol suggesting that the beta receptor may modulatory catecholamine stimulated transport. |
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