Molecular complexity of Paramecium symbiont lambda deoxyribonucleic acid: evidence for the presence of a multicopy genome |
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| Authors: | A T Soldo G A Godoy |
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| Affiliation: | Research Laboratories of the Veterans Administration Hospital and the Department of Biochemistry, University of Miami School of Medicine Miami, Fla 33125, U.S.A. |
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| Abstract: | DNA was isolated from highly purified symbiont lambda particles of Paramecium aurelia. Renaturation kinetic data revealed a molecular size of 0.71 × 109 daltons. Analytical complexity estimated from chemical data was 7.5 × 109 daltons. These values correspond to about ten copies of the genome per lambda particle. The relationship of symbiont lambda particles to bacteria and cellular organelles, i.e. chloroplasts, mitochondria and kinetosomes, is discussed. |
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| Keywords: | melting point (defined as that temperature at which half the total increase in absorbancy at 260 nm has occurred) |
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