Cloning genes from a library using a clustering strategy and PCR |
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| Authors: | S. Díaz Prado N. Tarrío M. E. Cerdán M. I. González Siso |
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| Affiliation: | 1. Department of Molecular and Cell Biology, University of A Coru?a, Campus da Zapateira s/n, 15071-A, Coru?a, Spain
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| Abstract: | ![]()
A new polymerase chain reaction (PCR)-based method is described for the isolation of clones of interest from a library when only part of a sequence is available. In actuality, this occurs with many genomes that have been partially sequenced using a random strategy. The method presented here, discriminating clusters by PCR (DCbyPCR), is a nonradioactive and improved alternative to colony hybridization. |
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| Keywords: | Cloning colon hybridization PCR |
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