龙须草花粉活性及结实率与其无融合生殖的关系

以两种生态型无融合生殖龙须草为材料，经去雄套袋、去掉柱头及相互杂交等处理，比较几种方式下的结实率。结果表明：去雄套袋、截去柱头以破坏授粉和杂交授粉等处理与去雄不套袋作对照之间结实率的差异不显著。两种生态型龙须草的成熟花粉粒都具有活力，在蔗糖溶液中的离体萌发率分别为28％和34％；花粉在柱头上的附着和生长的荧光观察表明：花粉附着率较低，花粉在柱头上可以萌发，但不能进入柱头和花柱。证明龙须草花粉对其结实率没有显著影响；龙须草具有很高的无融合生殖度，是优良的无融合生殖基因供体。

The Study and Analysis of Apomixis in Eulaliopsis binata

Two ecotypes of Eulaliopsis binata were used as materials to investigate the relationship between seed setting and apomictic traits. The flowers were pretreated by (A) emasculating then bagging,(B)removal of stigma and (C) crosspollination, respectively as compared with control: open-pollination after emasculation. The results indicated that pollination treatment did not show much influence on the seed set Pollen germination test revealed the pollen possessed normal activity (28% and 34% germination rate) while flowering and pollination, via fluorescent observation, the pollens of E. binata showed poor adherence to stigma. Though pollen grains could germinate on stigma but the pollen tube could not stretch into stigma further more. The above results concluded that E. binata could produce seed without involving of male gamete, their embryo and endosperm could develop autonomously without fertilization. As presented in has high degree of apomixis, E. binata might act as a potential valuable donor for apomixis gene.