The ruthenium complex <Emphasis Type="Italic">cis</Emphasis>-(dichloro)tetrammineruthenium(III) chloride induces apoptosis and damages DNA in murine sarcoma 180 cells |
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Authors: | Aliny Pereira de Lima Flavia de Castro Pereira Cesar Augusto Sam Tiago Vilanova-Costa Alessandra de Santana Braga Barbosa Ribeiro Luiz Alfredo Pavanin Wagner Batista Dos Santos Elisângela de Paula Silveira-Lacerda |
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Institution: | 1.Laboratório de Genética Molecular e Citogenética, Instituto de Ciências Biológicas,Universidade Federal de Goiás — UFG,Goiania, Goiás,Brazil;2.Instituto de Química,Universidade Federal de Uberlandia — UFU,Uberlandia, Minas Gerais,Brazil;3.Faculdade Patos de Minas — FPM,Patos de Minas,Minas Gerais,Brazil |
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Abstract: | Ruthenium(III) complexes are increasingly attracting the interest of researchers due to their promising pharmacological properties.
Recently, we reported that the cis-(dichloro)tetrammineruthenium(III) chloride compound has cytotoxic effects on murine sarcoma 180 (S-180) cells. In an effort
to understand the mechanism responsible for their cytotoxicity, study we investigated the genotoxicity, cell cycle distribution
and induction of apoptosis caused by cis-(dichloro)tetrammineruthenium(III) chloride in S-180 tumour cells. cis-(dichloro)tetrammineruthenium(III) chloride treatment induced significant DNA damage in S-180 cells, as detected by the alkaline
comet assay. In the cell cycle analysis, cis-(dichloro)tetrammineruthenium(III) chloride caused an increase in the number of cells in G1 phase, accompanied by a decrease
in the S and G2 phases after 24 h of treatment. In contrast, the cell cycle distribution of S-180 cells treated with cis-(dichloro)tetrammineruthenium(III) chloride for 48 h showed a concentration-dependent increase in the sub-G1 phase (indicating
apoptosis), with a corresponding decrease in cells in the G1, S and G2 phases. In addition, cis-(dichloro)tetrammineruthenium(III) chloride treatment induced apoptosis in a time-dependent manner, as observed by the increased
numbers of annexin V-positive cells. Taken together, these findings strongly demonstrate that DNA damage, cell cycle changes
and apoptosis may correlate with the cytotoxic effects of cis-(dichloro)tetrammineruthenium(III) chloride on S-180 cells. |
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