The incorporation of J chain into reassembled human immunoglobulin M |
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Authors: | Manuel J Ricardo Jr and Franklin P Inman |
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Institution: | Department of Microbiology, 831 Bio. Science Building, University of Georgia, Athens, Ga. 30602, U.S.A. |
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Abstract: | Human IgM (immunoglobulin M) was reduced with 24mm-mercaptoethylamine. This atreatment resulted in complete dissociation to IgMs subunits and free J chain. Intr-subunit interchain disulphide bonds remained intact. The mixture then was encouraged to reoxidize. The schlieren pattern of the reoxidized mixture showed the presence of a considerable quantity of IgM in addition to residual IgMs. The isolated reassembled IgM did not dissociate in 5m-guanidinium hydrochloride. It apparently contained the same amount of covalently attached J chain as did native IgM. The J chain was a part of the high-molecular-weight Fc fragment obtained from the reassembled IgM. |
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