兔多杀性巴氏杆菌铁调节外膜蛋白（IROMPs）抗原交叉性试验

分别以含铁培养基和限铁培养基培养4株兔多杀性巴氏杆菌JS、C51—2、C51-3及C51—17株，用刚果红结合试验初步分析铁调节外膜蛋白(IROMPs)表达情况，同时破碎菌体，提取外膜蛋白，经SDS-PAGE比较这4株细菌在正常培养条件、富铁培养条件及限铁培养条件时外膜蛋白的表达差异，结果表明：限铁培养时，细菌表现出对刚果红染料较强结合性，且这4株巴氏杆菌均表达数种高分子量的IROMPs，主要有147kD、135kD、99kD、94kD、82kD及72kD蛋白带，4株之间存在一定的差异，而正常或富铁条件培养时均不表达上述条带。免疫印迹结果显示，正常条件培养的全菌(C51—17株)抗血清中不含有针对IROMPs的抗体，限铁培养的C51-17株IROMPs可诱导机体产生相应抗体，并且能与JS、C51—2及C51-3株的IROMPs发生抗原交叉性反应。同时用间接ELISA检测C51—17株3种IROMP(99kD、94kD和87．6kD)的交叉抗体效价，结果这3种抗血清均与其它3株的产生较高的交叉抗体滴度。

Cross Antigenicity Test of Iron-regulated Outer Membrane Proteins (IROMPs) of Leporid Pasteurella multocida

Leporid Pasteurella multocida strain JS, C51-2, C51-3 and C51-17 were cultured in iron-replete, iron-restricted and normal conditions respectively. The bacterium cells grown under above conditions were measured to characterize iron-regulated outer membrane proteins (IROMPs) by the Congo Red binding test, all tested strains showed greater binding to Congo Red in case of the expression of IROMPs. The SDS-PAGE patterns of OMP extracts of these four strains grown under the iron-rich, iron-restricted or normal conditions, demonstrated that the IROMPs of 147kD,135kD,99kD,94kD,82kD and 72kD were expressed when grown in iron-restricted media, other than in iron-replete and normal conditions. Murine serum antibodies against the whole-cell bacterin of strain C51-17 grown in normal condition, couldn't react with the IROMPs. The IROMP extracts from strain C51-17 could elicit the hosts to produce antibodies, and antibodies could react with the IROMPs of the four strains by western blots. The bands of 99kD, 94kD or 87.6kD IROMP had the common antigens among the four strains in higher titers by ELISA. It showed that the IROMP extracts from P. multocida grown in iron-restricted condition, maybe had the epitopes of cross-protected factors (CPFs).