Effects of potato plants expressing a barley cystatin on the predatory bug Podisus maculiventris via herbivorous prey feeding on the plant |
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Authors: | Fernando Álvarez-Alfageme Manuel Martínez Sara Pascual-Ruiz Pedro Castañera Isabel Diaz Félix Ortego |
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Institution: | (1) Departamento de Biología de Plantas, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain;(2) Laboratorio de Bioquímica y Biología Molecular, Departamento de Biotecnología-UPM, E.T.S. Ingenieros Agrónomos, 28040 Madrid, Spain |
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Abstract: | The aim of this study was to assess the effects of potato plants expressing a barley cystatin on a potentially cystatin-susceptible
natural enemy by predation on susceptible and non-susceptible preys feeding on the plant. We have focussed on the impact of
the variant HvCPI-1 C68 → G, in which the only cysteine residue was changed by a glycine, on the growth and digestive physiology of the Colorado
potato beetle (CPB), Leptinotarsa decemlineata, and the Egyptian cotton leafworm (ECW), Spodoptera littoralis. Moreover, we have studied the prey-mediated effects of the barley cystatin at the third trophic level, using the predatory
spined soldier bug (SSB), Podisus maculiventris, as a model. Feeding trials conducted with CPB larvae reared on transgenic potato plants expressing the C68 → G variant resulted in significantly lower weight gains compared to those fed on non-transformed (NT) plants. On the contrary,
larger weight gains were obtained when ECW larvae, that lack digestive cysteine proteases, were reared on transgenic potato
expressing the cystatin, as compared to larvae fed on NT plants. No negative effects on survival and growth were observed
when SSB nymphs were exposed to HvCPI-1 C68 → G by predation on either CPB or ECW larvae reared on transgenic potato plants expressing the barley cystatin, despite the
fact that the inhibitor suppressed in vitro gut proteolysis of the predatory bug. To investigate the physiological background, biochemical analysis were carried out
on guts of insects dissected at the end of the feeding assays.
Fernando álvarez-Alfageme and Manuel Martínez are contributed equally to this work. |
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Keywords: | barley cystatin beneficial predators protease inhibitors tritrophic interactions |
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