A novel cysteine-rich antimicrobial peptide from the mucus of the snail of Achatina fulica |
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| Institution: | 1. Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China;2. The Affiliated Zhongda Hospital of Southeast University, Nanjing, Jiangsu, 210009, China;3. Nanjing Maternal and Child Health Hospital, 123# Tianfei Street, Baixia District, Nanjing, Jiangsu, 210004, China;1. Université de Tunis El Manar, Institut Pasteur de Tunis, Laboratoire des Venins et biomolécules thérapeutiques LR11IPT08/ LR16IPT08, Tunis, 1002, Tunisia;2. Université de Tunis El Manar, Institut Pasteur de Tunis, Laboratoire d''Epidémiologie Moléculaire et Pathologie Expérimentale appliquée aux Maladies infectieuses LR11IPT04/LR16IPT04, Tunis, 1002, Tunisia;3. Université de Tunis El Manar, Institut Pasteur de Tunis, Laboratoire de Recherche sur la Transmission, le Contrôle et l''Immunobiologie des Infections, LR11IPT02/ LR16IPT02 LTCII, Tunis, 1002, Tunisia;4. INSERM UMR 911-Centre de Recherche en Oncologie Biologique et Oncopharmacologie (CRO2), Aix-Marseille Université, 27 Bd Jean Moulin, 13385, Marseille Cedex 5, France;1. Department of Life Sciences, Via Licio Giorgieri 5, University of Trieste, 34127 Trieste, Italy;2. Department of Physics, Faculty of Science, University of Split, Soba B3-18, Ru?era Bo?kovi?a 33, 21000 Split, Croatia;3. Department of Medical Sciences, University of Trieste, Laboratorio Clinica Odontostomatologica, Piazza dell’Ospitale 1, 34125 Trieste, Italy;1. Department of Chemistry “G. Ciamician”, University of Bologna, Via Selmi 2, 40126, Italy;2. Department of Pharmacy and BioTechnology, University of Bologna, Via S. Donato 19/2, 40127, Italy;3. Department of Pharmacy and Biotechnology, University of Bologna, Via Massarenti 9, 40138, Italy;4. Microbiology Unit, St Orsola-Malpighi University Hospital, Via Massarenti 9, 40138 Bologna, Italy;1. Drug Delivery Research Unit, Department of Pharmaceutics, Faculty of Pharmaceutical Sciences University of Nigeria Nsukka, Enugu State, Nigeria;2. Instituto de Productos Naturales y Agrobiología del CSIC, Avda. Astrofísico Francisco Sánchez 3, 38206, La Laguna, Tenerife, Spain;3. Institute of Organic Chemistry, University of Regensburg, Universitätstrasse. 31, 93040, Regensburg, Germany;1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Yunnan Key Laboratory of Natural Pharmaceutical Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;2. School of Pharmacy, Yunnan University of TCM, Kunming 650500, China;3. School of Chemistry and Pharmacy, Dali University, Dali 671003, China |
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| Abstract: | Antimicrobial peptides (AMPs) are important components of the innate immunity. Many antimicrobial peptides have been found from marine mollusks. Little information about AMPs of mollusks living on land is available. A novel cysteine-rich antimicrobial peptide (mytimacin-AF) belonging to the peptide family of mytimacins was purified and characterized from the mucus of the snail of Achatina fulica. Its cDNA was also cloned from the cDNA library. Mytimacin-AF is composed of 80 amino acid residues including 10 cysteines. Mytimacin-AF showed potent antimicrobial activity against Gram-negative and Gram-positive bacteria and the fungus Candida albicans. Among tested microorganisms, it exerted strongest antimicrobial activity against Staphylococcus aureus with a minimal peptide concentration (MIC) of 1.9 μg/ml. Mytimacin-AF had little hemolytic activity against human blood red cells. The current work confirmed the presence of mytimacin-like antimicrobial peptide in land-living mollusks. |
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