The protective effect of oxytocin on ischemia/reperfusion injury in rat urinary bladder |
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| Institution: | 1. Acibadem University, School of Medicine, Department of Histology and Embryology, Istanbul, Turkiye;2. Istanbul Mehmet Akif Ersoy Thoracic and Cardiovascular Surgery Education and Research Hospital, Department of Cardiovascular Surgery, Istanbul, Turkiye;3. Istanbul Mehmet Akif Ersoy Thoracic and Cardiovascular Surgery Education and Research Hospital, Department of Biochemistry, Istanbul, Turkiye;4. Marmara University, School of Medicine, Department of Histology and Embryology, Uskudar, Istanbul, Turkiye;1. Tampere University Hospital, Critical Care Medicine Research Group, PO Box 2000, 33521 Tampere, Finland;2. Helsinki University Central Hospital, Lohja Hospital, Emergency Department, Sairaalatie 8, 08200 Lohja, Finland;3. Helsinki University Hospital, Department of Surgery, Division of Intensive Care Medicine, PO Box 340, 00029 Helsinki University Hospital, Finland;4. Kuopio University Hospital, Centre for Prehospital Emergency Care, PO Box 100, 70029 Kuopio University Hospital, Finland;5. Helsinki University Hospital, Jorvi Hospital, Department of Intensive Care, PO Box 800, 00029 Helsinki University Hospital, Finland;6. Helsinki University Hospital, Department of Neurology, PO Box 340, 00029 Helsinki University Hospital, Finland;7. University of Tampere, School of Health Sciences, 33014 University of Tampere, Finland;8. Kanta-Häme Central Hospital, Department of Intensive Care, Ahvenistontie 20, 13530 Hämeenlinna, Finland;9. South-Carelia Central Hospital, Department of Intensive Care, Valto Käkelänkatu 1, 53130 Lappeenranta, Finland;10. Lapland Central Hospital, Department of Intensive Care, PL 8041, 96101 Rovaniemi, Finland;11. Uppsala University Hospital, Department of Surgical Sciences, 75185 Uppsala, Sweden;1. Pôle de recherche en Endocrinologie, Diabète et Nutrition, Institut de Recherche Expérimentale et Clinique, Cliniques Universitaires St-Luc and Université Catholique de Louvain, Brussels, Belgium;2. Department of Laboratory Medicine, Cliniques Universitaires St-Luc and Université Catholique de Louvain, Brussels, Belgium;3. Division of Cardiology, Cliniques Universitaires St-Luc and Pôle de recherche cardiovasculaire, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium;3. From the Department of Pathology, Division of Neurosurgery, University of California at San Diego, La Jolla, California 92093;4. Department of Surgery, Division of Neurosurgery, University of California at San Diego, La Jolla, California 92093;1. College of Animal Science and Veterinary Medicine, Liaoning Medical University, Jinzhou 121001, Liaoning, PR China;2. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, Jilin, PR China |
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| Abstract: | Oxytocin (OXY), a well-known nonapeptide, plays a crucial role in reproduction, and has effects on modulating the immune and inflammatory processes in living organisms as well. Recently it is also known as an antioxidant in several organs. The present study aims to demonstrate the protective effect of OXY against ischemia/reperfusion (I/R) injury in urinary bladder tissue. Abdominal aorta of rats, were clamped to perform urinary bladder ischemia. OXY (0.5 μg/kg) was injected intraperitoneally before ischemia in I/R + OXY group, whereas the vehicle solution was injected to I/R group. At the end of reperfusion, tissue samples from urinary bladder were processed for histochemical, ultrastructural and biochemical analysis. Tissue sections were stained by toluidine blue for mast cell counting and hematoxylin–eosin for histopathology. In addition, malondialdehyde (MDA) and glutathione (GSH) levels were determined biochemically. The results demonstrated that there was an extreme damage at urothelium, dilatation of intercellular junctions, inflammatory cell infiltration in I/R group. I/R + OXY group demonstrated a reduction in the severity of urinary bladder damage. According to mast cell counting results, both granulated and degranulated mast cells were decreased in I/R + OXY group compared to I/R group. The mean MDA level was higher in I/R group compared to control and lower in I/R + OXY group compared to I/R group. GSH level reduced in I/R group compared to the control and increased in I/R + OXY group compared to I/R group. In conclusion, oxytocin, as confirmed by histological evaluation and biochemical assays has a potential protective effect in the urinary bladder tissue against ischemia/reperfusion injury. |
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