| 西伯利亚蓼中PsMnSOD基因的克隆及其抗逆性检测 |
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| 引用本文： | 付畅,刘关君,张恒,杨传平.西伯利亚蓼中PsMnSOD基因的克隆及其抗逆性检测[J].植物生理学通讯,2010(7):653-658. |
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| 作者姓名： | 付畅 刘关君 张恒 杨传平 |
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| 作者单位： | [1]哈尔滨师范大学生命科学与技术学院,哈尔滨150025 [2]东北林业大学林学院林木遗传育种与生物技术教育部重点实验室,哈尔滨150040 [3]东北林业大学林学院林木遗传育种黑龙江省重点实验室,哈尔滨150040 |
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| 基金项目： | 东北林业大学林木遗传育种与生物技术教育部重点实验室开放课题、东北林业大学林木遗传育种黑龙江省重点实验室开放课题; 黑龙江省青年科学技术专项资金项目（QC06C044）致谢StefanHohmann教授（Goteborg University,Sweden）馈赠酵母菌株w303-1A. |
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| 摘 要： | 采用RACE技术从西伯利亚蓼中克隆了锰超氧化物歧化酶基因PsMnSOD的cDNA（GenBank登录号FJ848572）。长为792bp的PsMnSODcDNA序列含有编码234个氨基酸的705bp的开放读码框、57bp的5＇非翻译区和30bp的3＇非翻译区。构建了PsMnSOD基因的酵母表达载体pYES2-PsMnSOD并将其转化到野生型酵母菌株中。分析PsMnSOD基因在酵母中的表达对酵母SOD酶活性的影响及其对盐胁迫、PEG胁迫、高温和低温胁迫下抗逆性影响的结果表明,PsMnSOD基因在酵母中表达后酵母SOD酶活性提高,酵母对盐渍、PEG、高温和低温等非生物胁迫的抗逆性也增强。
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| 关 键 词： | 西伯利亚蓼 锰超氧化物岐化酶 抗逆性 |
Cloning and Identification of Stress Resistance of Superoxide Dismutase Gene PsMnSOD from Polygonum sibiricum Laxm |
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| FU Chang,LIU Guan-Jun,ZHANG Heng,YANG Chuan-Ping.Cloning and Identification of Stress Resistance of Superoxide Dismutase Gene PsMnSOD from Polygonum sibiricum Laxm[J].Plant Physiology Communications,2010(7):653-658. |
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| Authors: | FU Chang LIU Guan-Jun ZHANG Heng YANG Chuan-Ping |
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| Institution: | 2,3,1College of Life Sciences and Technology,Harbin Normal University,Harbin 150025,China;2Key Laboratory of Ministry of Education for Forestry Tree Genetic Improvement and Biotechnology,3Key Laboratory of Heilongjiang Province for Forestry Tree Genetic Improvement,School of Forestry,Northeast Forestry University,Harbin 150040,China |
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| Abstract: | In this research,PsMnSOD cDNA （GenBank No.FJ848572） was isolated from Polygonum sibiricum by RACE technology.The nucleotide sequence of PsMnSOD cDNA is 792 bp containing an open reading frame of 705 bp encoding 234 amino acids,with a 5＇ untranslated region of 57 bp and a 3＇ untranslated region of 30 bp.The PsMnSOD gene yeast expression vector pYES2-PsMnSOD was constructed and transformed into wild yeast strain.The effects of PsMnSOD gene expression on superoxide dismutase activity and resistance to abiotic stress including salt,PEG,high temperature and low temperature of yeast were determined.The results indicated that expression of PsMnSOD gene in yeast improved superoxide dismutase activity and resistance ability to abiotic stress including salt,PEG,high temperature and low temperature. |
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| Keywords: | Polygonum sibiricum manganese superoxide dismutase stress resistance |
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