| Antiporter Gene from Hordum brevisubulatum （Trin.） Link and Its Overexpression in Transgenic Tobaccos |
| |
| 引用本文： | Shi-YouLU Yu-XiangJING Shi-HuaSHEN Hua-YanZHAO Lan-QingMA Xiang-JuanZHOU QingREN Yan-FangLI. Antiporter Gene from Hordum brevisubulatum （Trin.） Link and Its Overexpression in Transgenic Tobaccos[J]. 植物学报(英文版), 2005, 47(3): 343-349. DOI: 10.1111/j.1744-7909.2005.00027.x |
| |
| 作者姓名： | Shi-YouLU Yu-XiangJING Shi-HuaSHEN Hua-YanZHAO Lan-QingMA Xiang-JuanZHOU QingREN Yan-FangLI |
| |
| 作者单位： | [1]KeyLaboratoryofPlantPhotosynthesisandEnvironmentalMolecularPhysiology,InstituteofBotanytheChineseAcademyofSciences,Beijing100093,China [3]CollegeofPlantScience,JilinUniversity,Changchun130062,China |
| |
| 摘 要： | A vacuolar Na^ /H^ antiporter cDNA gene was successfully isolated fromHordeum brevisubulatum (Trin.) Link using the rapid amplification ofcDNA ends (RACE) method. The gene was named HbNHXI and was found to consist of 1 916 bp encoding a predicted polypeptide of 540 amino acids with a conserved amiloride-binding domain. Phylogenetic tree analysis of the Na^ /H^ antiporters showed that the HbNHXI gene shares 55.3%-74.8% similarity with the vacuolar-type Na^ /H^ antiporters. Transgenic tobaccos that contain the HbNHXI gene, integrated by forward insertion into the tobacco genome, were obtained via Agrobacterium tumerfaciens and characterized for the determination of the concentration of Na^ and K^ ions, as well as proline, in the presence of 300 mmol/L NaCl. The T1 transgenic plants showed more tolerance to salt and drought than did wild-type plants. Our data suggest that overexpression of the HbNHXI gene could improve the tolerance of transgenic tobaccos to salt and drought through the function of the vacuolar Na^ /H^ antiporter.
|
| 关 键 词： | 转基因烟草 过表达 HbNHXL基因 Hordum brevisubulatum （Trin.） 盐胁迫 钠离子／氢离子转运 |
Antiporter Gene from Hordum brevisubulatum (Trin.) Link and Its Overexpression in Transgenic Tobaccos |
| |
| Shi-You L,Uuml;,Yu-Xiang JING,Shi-Hua SHEN,Hua-Yan ZHAO,Lan-Qing MA,Xiang-Juan ZHOU,Qing REN,Yan-Fang LI. Antiporter Gene from Hordum brevisubulatum (Trin.) Link and Its Overexpression in Transgenic Tobaccos[J]. Journal of integrative plant biology, 2005, 47(3): 343-349. DOI: 10.1111/j.1744-7909.2005.00027.x |
| |
| Authors: | Shi-You LÜ ,Yu-Xiang JING,Shi-Hua SHEN,Hua-Yan ZHAO,Lan-Qing MA,Xiang-Juan ZHOU,Qing REN,Yan-Fang LI |
| |
| Affiliation: | College of Plant Science, Jilin University, Changchun 130062, China;; Key Laboratory of Plant Photosynthesis and Environmental Molecular Physiology, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China |
| |
| Abstract: | Abstract: A vacuolar Na+/H+ antiporter cDNA gene was successfully isolated from Hordeum brevisubulatum (Trin.) Link using the rapid amplification of cDNA ends (RACE) method. The gene was named HbNHX1 and was found to consist of 1 916 bp encoding a predicted polypeptide of 540 amino acids with a conserved amiloride-binding domain. Phylogenetic tree analysis of the Na+/H+ antiporters showed that the HbNHX1 gene shares 55.3%–74.8% similarity with the vacuolar-type Na+/H+ antiporters. Transgenic tobaccos that contain the HbNHX1 gene, integrated by forward insertion into the tobacco genome, were obtained via Agrobacterium tumerfaciens and characterized for the determination of the concentration of Na+ and K+ ions, as well as proline, in the presence of 300 mmol/L NaCl. The T1 transgenic plants showed more tolerance to salt and drought than did wild-type plants. Our data suggest that overexpression of the HbNHX1 gene could improve the tolerance of transgenic tobaccos to salt and drought through the function of the vacuolar Na+/H+ antiporter.
(Managing editor: Li-Hui ZHAO) |
| |
| Keywords: | HbNHX1 gene Hordeum brevisubulatum (Trin.) Link salt stress tobacco vacuolar Na+/H+ antiporter |
| 本文献已被 维普 等数据库收录！ |
|
