梨遗传连锁图谱的构建及其与苹果图谱的比较

以‘丰水’为母本、‘砀山酥梨’为父本杂交所得的F1代104株单体为作图群体,利用SSR分子标记进行遗传连锁分析,应用Jionmap 3.0作图软件,构建了一张包含104个SSR分子标记,分属于18个连锁群的梨遗传连锁图谱,覆盖梨基因组总长831.8cM,平均图距为8.0cM。根据定位到该图谱上的SSR标记与苹果‘Fiesta’图谱进行比较,25个共有的SSR标记将该图谱和苹果图谱各连锁群连接起来,这些标记不仅呈现良好的共线性而且它们之间的相对遗传距离也很相近。研究认为,SSR标记作为锚定引物,可以与不同物种的遗传图谱相比较整合,为不同物种之间遗传信息的转移提供参考依据;同时该研究为梨树相关性状的基因定位、分离以及克隆奠定了基础。

Construction of a Genetic Linkage Map in Pear and Compared the Map with Apples

Based on the SSR molecular marker and Jionmap 3.0 analysis a genetic linkage map of pear was constructed with F1 populations from a cross of ’Housui’ and ’Dangshansuli’.The map consisted of 18 linkage groups and located 104 SSR markers covered 831.8 cM of pear genomic with an average distance of 8.0 cM per markers.According to the 25 commom SSRs marked on this pear map and the apple ’Fiesta’ map,the homologous linkage groups were well connected to each other and it showed high level of co-linearity between the two maps.SSR markers on the map can be taken as anchor points to be merged with other maps.