鹤顶兰种子萌发及原球茎增殖培养研究

以鹤顶兰成熟蒴果的种子为实验材料,研究影响种子非共生萌发的因素和种胚发育途径,并采用正交设计研究了原球茎增殖的影响因素。结果表明:冷藏影响种子的活力且其萌发率随冷藏时间延长而降低;0.5%NaClO溶液浸泡种子可提高萌发率,缩短初始萌发时间;种胚发育途径为种胚转绿后从种子侧面突破种皮而形成原球茎,随后分化出具根芽结构的完整植株;6-BA对原球茎增殖作用显著,原球茎增殖的最适培养基为1/2MS+3.0mg/L6-BA+1.0mg/L KT+1.0mg/L NAA,增殖倍数为6.67。

Study on Seed Germination and the Protocorm Multiplication of Phaius tankervilleae

In this study,the germination of the seeds in mature capsules of Phaius tankervilleae and the developmental pathway of its protocorms were investigated.The orthogonal design was utilized to study the effect factors on multiplication of protocorms.The results showed that the seeds germination rate decreased during low temperature storage.The more the cold storage time was prolonged,the more the seeds germination rate was declined.In addition,seeds treated with 0.5% NaClO,had a higher germination rate and needed a shorter initial germination time compared to the control.The developmental pathway of embryo was as following:embryo in seed turn green before breaking through the seed coat,then broke the seed coat from one side of the seed and turn protocorms,the protocorms then developed into the seedlings.6-BA had a significant effect on the multiplication of protocorms and the optimum culture medium for multiplication of protocorms was 1/2MS+3.0 mg/L 6-BA+1.0 mg/L KT+1.0 mg/L NAA,and the mean generation multiple was 6.67.