甘草根中甘草酸的免疫组织化学定位研究

应用免疫组织化学定位和HPLC分析方法,对人工种植乌拉尔甘草根中的甘草酸分布和积累变化规律进行分析,以阐明药用甘草根在发育过程中甘草酸的积累变化规律,探讨甘草药材质量形成的内在机制。结果表明:(1)甘草酸主要分布在根的次生韧皮部薄壁细胞和维管射线细胞中,且主要积累在这些细胞的细胞壁上。(2)HPLC分析显示,主根的韧皮部中甘草酸含量最高,其次是木质部,根皮中含量最少。(3)甘草酸在主根中的含量随着生长年限的增加而提高,2、3年生甘草根中甘草酸含量上升较快,3年后甘草酸的含量达到3.66%,超过了国家药典规定的甘草酸含量标准。

Immunohistochemical Localization of Glycyrrhizic Acid in the Radix of Glycyrrhiza uralensis Fisch.

The accumulation,distribution and content changes of glycyrrhizic acid of a medicinal licorice Glycyrrhiza uralensis Fisch.were investigated by means of immunohistochemical localization and HPLC methods.The results showed that:(1)The glycyrrhizic acid distributed mainly in the cell wall of phloem parenchyma cells and ray cells of the roots;(2)The content of glycyrrhizic acid in different tissues of taproot was varied.The highest one was in phloem and then in xylem,while the lowest in the root bark.(3)The content of glycyrrhizic acid tended to increase with the growth years in the roots.It increased quickly in 2-years and 3-yesars and the content of glycyrrhizic acid is 3.66% in 3-yaers,which exceeds the standard of Chinese pharmacopoeia.The results provide a further understanding on the internal mechanism of the quality formation of medicinal licorice and to improve quality of medicine licorice.