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Effects of glucocorticoids on the respiratory burst of Chlamydia-primed THP-1 cells |
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| Authors: | Mouithys-Mickalad Ange Deby-Dupont Ginette Mathy-Hartert Marianne Habraken Yvette Nys Monique Henrotin Yves Lamy Maurice Deby Carol |
| Institution: | a Centre for Oxygen, Research and Development (CORD), Institut de Chimie, B6a, University of Liège, Sart Tilman, 4000 Liège, Belgium b Department of Anaesthesia and Intensive Care, University Hospital of Liège, Belgium c Bone and Cartilage Metabolism Research Unit, University of Liège, Belgium d Laboratory of Fundamental Virology, University of Liège, Belgium |
| Abstract: | We previously observed that the respiratory burst of human monocytes (THP-1 cell line) triggered by phorbol myristate acetate was strongly enhanced by a priming of the cells by Chlamydia pneumoniae Biochem. Biophys. Res. Commun. 287 (2001) 781]. We describe here the modifications of the responses of Chlamydia-primed THP-1 cells to hydrocortisone (HCT) and methylprednisolone (MPL). HCT and MPL inhibited the production of the cytokines TNFα and IL-8. But HCT, which inhibited the respiratory burst in LPS-primed monocytes, paradoxically stimulated the phenomenon in Chlamydia-primed cells; MPL exerted no significant effect. Both glucocorticoids did not significantly modify the triggering effect of Chlamydia on NF-κB binding activity. On the expression of p22phox, a protein subunit of the NADPH oxidase, HCT had an increasing and MPL a decreasing effect. Glucocorticoids thus had unexpected effects on the inflammatory response of Chlamydia-primed monocytes. |
| Keywords: | Monocytes Chlamydia pneumoniae Hydrocortisone Methylprednisolone NADPH-oxidase IL-8 TNFα NF-κB Priming |
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