Relationship between colonial surface and density on agar plate |
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Authors: | C Chapuis L Rosso J P Flandrois |
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Institution: | Facultéde médecine Lyon-Sud, Laboratoire de Bactériologie, CNR URA 2055, Oullins;Laboratoire de Biométrie, CNRS URA 2055, UniversitéClaude Bernard, Villeurbanne;CREALIS, DANONE, Inc., Brive, France |
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Abstract: | The size of a colony on an agar plate is influenced by the number of colonies ( N ) on this plate. When N is small, colonies reach a larger size. The relationship between colonial surface and density of bacteria on agar plate was studied for nine bacterial and two yeast strains. A mathematical model describing the relationship between the logarithm base 10 of the number of colonies on the agar plate and the average colonial diameter was built. This model is shown to be adapted to most of the strains studied and could be a tool for media quality control. |
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