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Novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sections |
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| Authors: | Keiichi Hatakeyama Kanako Wakabayashi-Nakao Yutaka Aoki Shun-ichiro Ogura Ken Yamaguchi Takashi Nakajima Taka-Aki Sato Tohru Mochizuki Isamu Hayashi |
| Affiliation: | 1. Medical Genetics Division, Shizuoka Cancer Center Research Institute, 1007 Shimonagakubo, Nagaizumi-cho, Sunto-gun, Shizuoka, 411-8777, Japan 2. Life Science Research Center, Shimadzu Corporation, 1 Nishinokyo-Kuwabara-cho, Nakagyo-ku, Kyoto, 604-8511, Japan 3. Frontier Research Center, Tokyo Institute of Technology, 4259-B102 Nagatsuta-cho, Midori-ku, Yokohama, 226-8051, Japan 4. Shizuoka Cancer Center Hospital and Research Institute, 1007 Shimonagakubo, Nagaizumi-cho, Sunto-gun, Shizuoka, 411-8777, Japan 5. Pathology Division, Shizuoka Cancer Center Hospital, 1007 Shimonagakubo, Nagaizumi-cho, Sunto-gun, Shizuoka, 411-8777, Japan |
| Abstract: | We describe a novel antigen-retrieval method using a micro-sized chamber for mass spectrometry (MS) analysis to identify proteins that are preferentially eluted from formalin-fixed paraffin-embedded (FFPE) samples. This approach revealed that heat-induced antigen retrieval (HIAR) from an FFPE sample fixed on a glass slide not only improves protein identification, but also facilitates preferential elution of protein subsets corresponding to the properties of antigen-retrieval buffers. Our approach may contribute to an understanding of the mechanism of HIAR. |
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