去卵巢骨质疏松山羊骨髓基质细胞成骨分化能力的研究

目的：研究在构建的去卵巢骨质疏松山羊动物模型中，骨髓基质细胞（MSCs）的生物学特性以及其成骨能力。方法：建立去卵巢骨质疏松山羊动物模型，使用全骨髓法获取去卵巢骨质疏松山羊（实验组）和正常山羊（对照组）MSCs，流式细胞仪检测实验组和对照组细胞周期及增殖指数（PI）；地塞米松诱导21d时油红O染色，观察成脂分化比例；成骨诱导液诱导14d，碱性磷酸酶（ALP）染色、检测ALP表达量。结果：对照组PI高于实验组；地塞米松诱导后实验组脂肪细胞比例明显高于对照组；成骨诱导第7d，对照组ALP的表达量明显高于实验组。结论：去卵巢骨质疏松山羊的MSCs增殖和成骨分化能力都降低，可能与骨质疏松症的发病机理有关。

Study of osteogenic potential of ovariectomied goat MSCs

Objective:To investigate the growth characteristic of ovariectemied osteoporotic goat bone marrow stromal stem cells and its osteogenic capability.Method:We established the animal model of osteoporosis by performing ovafiectomy on the 2-yeal-old female goat. MSCs were isolated by all bone marrow culture methed.Cell cycle and proliferation index were detected by flow cytometry of the 3rd passage MSCs of both two groups.The 2nd passage MSCs were induced by dexamethasone of 10~(-7)mol/L and osteogenic inducer for 21days.The differentiation of adipocytes or osteoblasts were observed by oil red O and ALP staining.Results:PI of MSCs was lower in MSCs ovx group than in the MSCs control group.After 21 days of dexamethsone inducing,the ratio of the MSCs which displayed accumulation of lipid vacuoles were 76.6% in ovx group and 71.4% in control group.After 12 days of osteogenic inducing,the rates of ALP positive cells were 76.2% and 81.3% separately. Conclusions:The proliferation potential and osteogenic capacity of MSCs of ovariectomied osteoporotic goat were decreased,that may be the cel- lular mechanism of osteoporesis.