Hyphal walls of isolated lichen fungi |
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Authors: | Margalith Galun Amnon Braun Asher Frensdorff Esra Galun |
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Institution: | (1) Department of Botany, The Dr. George S. Wise Center for Life Sciences, Tel Aviv University, Tel Aviv, Israel;(2) Department of Microbiology, The Dr. George S. Wise Center for Life Sciences, Tel Aviv University, Tel Aviv, Israel;(3) Department of Plant Genetics, The Weizmann Institute of Science, Rehovoth, Israel |
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Abstract: | The hyphal walls of three mycobionts, isolated from the lichens Xanthoria parietina, Tornabenia intricata and Sarcogyne sp. were investigated by two techniques: microautoradiography of fungal colonies exposed to radioactive carbohydrate precursors; and binding, in vivo, of fluorescein conjugated lectins to hyphal walls of such colonies.N-3H] acetylglucosamine was readily incorporated into tips, young hyphal walls and septa of the three mycobionts and the free-living fungus Trichoderma viride, but not into Phytophthora citrophthora, indicating that chitin is a major component of the mycobionts' hyphal walls. All three mycobionts, but neither of the free-living fungi, incorporated 3H] mannose and 3H] mannitol into their hyphal walls.Fluorescein-conjugated wheat germ agglutinin was bound to the hyphal walls of the three mycobionts and T. viride, but not to the walls of P. citrophthora; the binding pattern was similar to the grain pattern obtained in autoradiographs after short N-3H] acetylglucosamine labelling. As wheat germ agglutinin binds specifically to chitin oligomers, the lectin binding tests further confirmed that chitin is a mycobiont hyphal wall component.Binding characteristics of several fluorescein-conjugated lectins to the three mycobionts indicated that this technique can yield useful information concerning the chemical composition of hyphal wall surfaces.List of abbreviations FITC
fluorescein isothiocyanate
- WGA
wheat germ agglutinin
- TCA
trichloroacetic acid
- PNA
peanut agglutinin
- LA
lotus agglutinin
- Glc NAc
N-acetylglucosamine
- ConA
concanavalin A
- SBA
soybean agglutinin
- WBA
waxbean agglutinin
Part of an M.Sc. thesis submitted by A. Braun to the Department of Botany, Tel Aviv University. |
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Keywords: | Precursor incorporation Fluorescein-conjugated lectins Lichen fungus cultures Cell wall components Chitin Xanthoria parietina Tornabenia intricata Sarcogyne sp Trichoderma viride Phytophthora citrophthora |
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