小麦耐盐突变体盐胁迫下的蛋白质组分析

首次采用双向电泳的方法分析1％NaCl胁迫72h的一对小麦耐盐(RH8706-49)及敏盐突变体(H8706-34)的蛋白质组。经过MALDI-TOF分析和数据库检索发现两者在H^ -ATP酶β亚基、谷氨酰胺合成酶前体、OEC33和RuBP羧化酶小亚基等5个候选蛋白存在质或量的差异。这5种蛋白均为叶绿体蛋白，它们很可能在盐胁迫下对维持叶绿体及整个细胞的功能起到重要作用。

Proteomic Analysis of the Salt Tolerance Mutant of Wheat Under Salt Stress

Two dimensional electrophoresis was used to analyse the proteome of the salt-tolerant mutant of wheat (RH8706-49) and the salt-sensitive mutant of wheat (H8706-34) which had been treated by 1% NaCl for 72 hours.After being analysised by MALDI-TOF-MS and Mascot software,the qualitative and quantitative differences were identified between the two materials for five candidate proteins:H~ -transporting two-sector (ATPase),glutamine synthetase 2 precursor, putative 33 kD oxygen evolvingprotein of photosystem II and ribulose-1,5-bisphos-phate carboxylase/oxygenase small subunit.These five proteins are all belong to chloroplast proteins.They are likely to play a crucial role in keeping the function of the chloroplast and the whole cells when the plant was under salt-stress.