Cloning and expression of tryptophan genes from Brevibacterium lactofermentum in Escherichia coli |
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| Authors: | G del Real A Aguilar J F Martín |
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| Affiliation: | 1. School of Life Sciences and Technology, Harbin Institute of Technology, Harbin, 150000, China;2. Faculty of Science, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, 10520, Thailand;1. Department of Chemistry and Bioscience, Aalborg University, Niels Bohrs Vej 8, 6700 Esbjerg, Denmark;2. Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, 9220 Aalborg, Denmark;3. Commonwealth Scientific and Industrial Research Organization (CSIRO) Agriculture and Food, Queensland Bioscience Precinct, 306 Carmody Rd, St. Lucia, QLD 4067, Brisbane, Australia;1. Lignocellulose Biotechnology Lab, Department of Microbiology, University of Delhi South Campus, New Delhi 110021, India;2. Product Development Cell, National Institute of Immunology, New Delhi 110067, India |
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| Abstract: | A gene bank from the amino acid producer Brevibacterium lactofermentum has been prepared in Escherichia coli using pBR322 as vector. Four clones containing genetic information needed to complement mutations in A,B,C and D genes from E. coli have been isolated. The cloned fragments range between 4.3 kb (pULT61) and 7.9 kb (pULT62). All the four clones contain genetic information that complements trpB gene from E. coli. The cloned trpB gene is very stable and is maintained extrachromosomally in E. coli. It is expressed very efficiently showing high levels of tryptophan synthetase activity. |
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