Purification and characterization of restriction endonuclease BcoI from a soil isolate of Bacillus coagulans |
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| Authors: | S.M. Leung K.M. Kam K.Y. Chan P.C. Shaw |
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| Affiliation: | Department of Biochemistry, Sai Ying Pun Jockey Club Clinic, Hong Kong;Department of Biology, The Chinese University of Hong Kong, Sai Ying Pun Jockey Club Clinic, Hong Kong;Institute Pathology, Sai Ying Pun Jockey Club Clinic, Hong Kong |
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| Abstract: | Abstract A soil identified as Bacillus coagulans is found to produce Bco I, an isoschizomer of Ava I and with the same cleavage site. This thermal stable enzyme, Bco I, is produced at high level and can be isolated by passing the crude bacterial lysate through a DEAE-cellulose column. |
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| Keywords: | Type II restriction endonuclease AvaI Isoschizomer |
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