The oligodeoxynucleotide sequences corresponding to never-expressed peptide motifs are mainly located in the non-coding strand |
| |
Authors: | Giovanni Capone Giuseppe Novello Candida Fasano Brett Trost Mik Bickis Anthony Kusalik Darja Kanduc |
| |
Institution: | (1) Department of Biochemistry and Molecular Biology "Ernesto Quagliariello", University of Bari, Bari, Italy;(2) Department of Computer Science, University of Saskatchewan, Saskatoon, Canada;(3) Department of Mathematics and Statistics, University of Saskatchewan, Saskatoon, Canada |
| |
Abstract: | Background We study the usage of specific peptide platforms in protein composition. Using the pentapeptide as a unit of length, we find
that in the universal proteome many pentapeptides are heavily repeated (even thousands of times), whereas some are quite rare,
and a small number do not appear at all. To understand the physico-chemical-biological basis underlying peptide usage at the
proteomic level, in this study we analyse the energetic costs for the synthesis of rare and never-expressed versus frequent
pentapeptides. In addition, we explore residue bulkiness, hydrophobicity, and codon number as factors able to modulate specific
peptide frequencies. Then, the possible influence of amino acid composition is investigated in zero- and high-frequency pentapeptide
sets by analysing the frequencies of the corresponding inverse-sequence pentapeptides. As a final step, we analyse the pentadecamer
oligodeoxynucleotide sequences corresponding to the never-expressed pentapeptides. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|