All <Emphasis Type="Italic">Trypanosoma cruzi</Emphasis> developmental forms present lysosome-related organelles |
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Authors: | Celso Sant’Anna Fabiola Parussini Daniela Lourenço Wanderley de Souza Juan Jose Cazzulo Narcisa Leal Cunha-e-Silva |
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Institution: | 1.Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho,Universidade Federal do Rio de Janeiro,Ilha do Fund?o, Rio de Janeiro,Brazil;2.Diretoria de Programas (DIPRO), INMETRO, Rio de Janeiro,Brazil;3.Instituto de Investigaciones Biotecnológicas, Instituto Tecnológico de Chascomús,Universidad Nacional de General San Martín, CONICET,San Martín,Argentina;4.Microbiology and Molecular Genetics,University of Vermont,Burlington,USA |
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Abstract: | Trypanosoma cruzi epimastigote forms concentrate their major protease, cruzipain, in the same compartment where these parasites store macromolecules
obtained from medium and for this ability these organelles were named as reservosomes. Intracellular digestion occurs mainly
inside reservosomes and seems to be modulated by cruzipain and its natural inhibitor chagasin that also concentrates in reservosomes.
T. cruzi mammalian forms, trypomastigotes and amastigotes, are unable to capture macromolecules by endocytosis, but also express cruzipain
and chagasin, whose role in infectivity has been described. In this paper, we demonstrate that trypomastigotes and amastigotes
also concentrate cruzipain, chagasin as well as serine carboxypeptidase in hydrolase-rich compartments of acidic nature. The
presence of P-type proton ATPase indicates that this compartment is acidified by the same enzyme as epimastigote endocytic
compartments. Electron microscopy analyzes showed that these organelles are placed at the posterior region of the parasite
body, are single membrane bound and possess an electron-dense matrix with electronlucent inclusions. Three-dimensional reconstruction
showed that these compartments have different size and shape in trypomastigotes and amastigotes. Based on these evidences,
we suggest that all T. cruzi developmental stages present lysosome-related organelles that in epimastigotes have the additional and unique ability of
storing cargo. |
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Keywords: | Lysosome-related organelles Reservosomes Serine carboxypeptidase Trypomastigotes Amastigotes Trypanosoma cruzi |
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