The fluidity of the nuclear envelope lipid does not affect the rate of nucleocytoplasmic RNA transport in mammalian liver |
| |
Authors: | Paul S Agutter Keith E Suckling |
| |
Institution: | 1. Department of Biological Sciences, Napier College, Colinton Road, Edinburgh EH10 5DT U.K.;2. Department of Biochemistry, Hugh Robson Building, University of Edinburgh, George Square, Edinburgh, EH8 9XD U.K. |
| |
Abstract: | The effects of in vitro and in vivo modifications of nuclear envelope lipid on DNA leakage and on ATP-stimulated RNA release from isolated rat liver nuclei were investigated. The modifications included corn-oil feeding of the animals to alter the fatty acid composition of the lipids, phospholipase treatment of the isolated nuclei, and extraction of the total lipid with Triton X-100. Significant changes in lipid composition and approximate order parameter values of the spin-label 5-doxylstearate resulted, but there was no significant effect on RNA transport rate. It was concluded that the nuclear envelope lipid does not play any important part in nucleocytoplasmic RNA transport in mammalian liver. |
| |
Keywords: | RNA transport Nuclear envelope Nuclear membrane lipid (Rat liver) |
本文献已被 ScienceDirect 等数据库收录! |
|