Stabilization of 30 S ribosomal subunits of Bacillus subtilis W168 by spermidine and magnesium ions |
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Authors: | Kazuei Igarashi Tomohito Kakegawa Seiyu Hirose |
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Institution: | Faculty of Pharmaceutical Sciences, Chiba University, Yayoi-cho, Chiba, Chiba 260 Japan |
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Abstract: | An explanation for the fragility of 30 S ribosomal subunits of Bacillus subtilis has been studied. Degradation of 16 S ribosomal RNA, rather than degradation of ribosomal proteins, was found to cause the inactivation of 30 S subunits. Although RNAases were bound specifically to 30 S ribosomal subunits, the RNAases were able to function. Spermidine was found to contribute to the stabilization of 30 S ribosomal subunits by inhibiting the degradation of 16 S ribosomal RNA. A high concentration of Mg2+ also stabilized the 30 S ribosomal subunits of Bacillus subtilis. The polypeptide synthetic activity of 30 S ribosomal subunits prepared in the presence of spermidine was at least 4-times greater than that of 30 S ribosomal subunits prepared in the absence of spermidine; this activity was maintained without any loss for 3 months at ?70°C. |
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Keywords: | Ribosome stabilization 30 S subunit Spermidine (B subtilis) |
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