睡眠剥夺对大鼠行为学及咀嚼肌肌电图的影响

目的：颞下颌关节紊乱病是口腔科的一种常见病和多发病，精神心理因素是颞下颌关节紊乱病的一个主要病因。本文通过观察睡眠剥夺对大鼠行为学及咀嚼肌肌电图的影响，探讨睡眠剥夺在颞下颌关节紊乱病发病中的作用。方法：35只Wistar大鼠，随机分为5组：睡眠剥夺1d组、5d组、9d组、正常对照组和大平台对照组。采用改良多平台睡眠剥夺法（modified multiple plat—formmethod，MMPM）建立大鼠SD模型，观察大鼠行为学及咀嚼肌肌电图的变化。结果：睡眠剥夺1d组和5d组在旷场实验水平得分和垂直得分上均高于对照组，而睡眠剥夺9d组均低于对照组；睡眠剥夺1d、5d和9d组在松弛状态和紧咬状态时颞肌前后束及咬肌的电位均明显高于对照组，且两侧无明显差别，同时，睡眠剥夺组双侧颞肌和咬肌的肌电图静息期较对照组显著延长。结论：睡眠剥夺可使大鼠行为学发生改变并对咀嚼肌肌电图造成影响，这可能是颞下颌关节紊乱病的病因之一，为我们对颞下颌关节紊乱病的预防和治疗提供了一定的理论指导。

Effect of Sleep Deprivation on Praxiology and Electromyogram in Rat

Objective： Temporomandibular joint disorders （TMD） is a common and frequently-occurring disease in Department of Stomatology, and psychological factor is one of the main causes of the disease. This article to observe the influence of sleep deprivation upon the praxiology and electromyography （EMG） of masticatory muscle, and to investigate the effect of sleep deprivation upon the pathogenesis of TMD. Methods： 35 Wistar rats were randomly divided into five groups： normal cage group, tank cage group, SDld group, SD5d group, and SD9d group. Then ＂modified multiple plat-form method＂ was used to develop rat sleep deprivation model followed by detection ofpraxiology and electromyography of masticatory muscle. Results： In the open-field test, level scores and vertical scores of SD ld and 5d group were higher than that of control group, and SD 9d group was lower than control group. The electric potentials of temporalis and masseter at both lax and biting status 1, 5 and 9 days after model establishment were significantly higher compared with those of control group. No evident difference was found between bilateral sides. Meanwhile, the resting periods of bilateral temporalis and masseter in sleep deprivation group, as shown on EMG, were significantly prolonged compared with those in control group. Conclusion： Sleep deprivation is able to affect the praxiology and EMG of masticatory muscle, serving as a potential etiology of TMD. This will provide some theoretical guidance for the prevention and treatment of TMD.