Virulence Factors Produced by Staphylococcus aureus Biofilms Have a Moonlighting Function Contributing to Biofilm Integrity
Institution:
From the 3Institute of Microbiology, Department of Microbial Physiology and Molecular Biology;;4Institute of Biochemistry, Department of Cellular Biochemistry and Metabolomics;;5Institute of Microbiology, Department of Microbial Proteomics; University of Greifswald, Germany
Abstract:
Highlights
?Establishment of a flow system allowing multi-omics analysis of S. aureus biofilms.
?Biofilm proteome profiling (intracellular and ECM) plus metabolic footprint analysis.
?Virulence factors and ribosomal proteins stabilize the ECM as moonlighting proteins.
?They act as electrostatic bridges between anionic cell surfaces, eDNA and metabolites.