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Streamlined Protocol for Deep Proteomic Profiling of FAC-sorted Cells and Its Application to Freshly Isolated Murine Immune Cells*
Affiliation:From the 3The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142;;4Klarman Cell Observatory, The Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142;;5Division of Immunology, Department of Microbiology and Immunobiology, Harvard Medical School, and Evergrande Center for Immunologic Diseases, Harvard Medical School and Brigham and Women''s Hospital, Boston, Massachusetts;;6Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts 02215;;**Center for Cancer Immunology, Massachusetts General Hospital, Boston, Massachusetts 02114;;12Howard Hughes Medical Institute and Koch Institute for Integrative Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02140
Abstract:
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