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Precision De Novo Peptide Sequencing Using Mirror Proteases of Ac-LysargiNase and Trypsin for Large-scale Proteomics
Institution:3. From the 3Key Lab of Intelligent Information Processing of Chinese Academy of Sciences (CAS), Institute of Computing Technology, CAS; University of Chinese Academy of Sciences; Institute of Computing Technology, CAS, Beijing 100190, China;;4. 4State Key Laboratory of Proteomics; Beijing Proteome Research Center; National Center for Protein Sciences Beijing; Beijing Institute of Lifeomics, Beijing 102206, China;;5. 5Key Laboratory of Combinatorial Biosynthesis and Drug Discovery of Ministry of Education Wuhan University, Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, China;;6. 6Centre for Blood Research, University of British Columbia, Vancouver, British Columbia, Canada;
Abstract:
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  • Highlights
    • ?The developed Ac-LysargiNase showed higher stability and activity than before.
    • ?The merged spectra of the mirror peptides achieved nearly complete ion coverage.
    • ?pNovoM obviously increased the efficiency and accuracy of peptide sequencing.
    • ?The mirror enzymatic strategy achieved precision de novo sequencing on proteome scales.
    Keywords:De novo sequencing  Peptide mass fingerprinting  Mass Spectrometry  Protein engineering  Enzyme catalysis*  Ac-LysargiNase  Mirror proteases  Trypsin
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