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Integration of Two In-depth Quantitative Proteomics Approaches Determines the Kallikrein-related Peptidase 7 (KLK7) Degradome in Ovarian Cancer Cell Secretome
Institution:From the 3Queensland University of Technology (QUT), Institute of Health and Biomedical Innovation (IHBI) and School of Biomedical Sciences at the Translational Research Institute, 37 Kent Street, Woolloongabba, Queensland, 4102, Australia;;4Protein Discovery Centre, QIMR Berghofer Medical Research Institute, 300 Herston Road, Herston, Queensland, 4006, Australia;;5Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Victoria, Australia 3800;;6Klinische Forschergruppe der Frauenklinik, Klinikum Rechts der Isar, TU München, Munich, Germany
Abstract:
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  • Highlights
    • ?Kallikrein-related peptidase 7 is over expressed in ovarian cancer.
    • ?Quantitative PROTOMAP and TAILS approaches identified putative substrates of KLK7.
    • ?Pro-MMP10 is activated by KLK7.
    • ?KLK7 cleaves thrombospondin 1 and IGFBP6 in vitro.
    Keywords:Degradomics*  Extracellular matrix*  Proteases*  Ovarian cancer  Secretome  Substrate identification  Kallikrein-related Peptidases
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