A rapid method for the purification of D-amino acid oxidase of Trigonopsis variabilis by hydrophobic chromatography |
| |
| Authors: | Ashwini Deshpande K. Sankaran S. F. D'Souza G. B. Nadkarni |
| |
| Affiliation: | (1) Food Technology & Enzyme Engineering Division, Bhabha Atomic Research Centre, 400 085 Bombay, India |
| |
| Abstract: | Summary A relatively simple method has been described for the rapid purification of D-amino acid oxidase from Trigonopsis variabilis by hydrophobic chromatography on Phenyl-Sepharose CL-4B and negative adsorption on DEAE-cellulose. The purified enzyme had a specific activity of 22–24 units at 25°C and exhibited three bands on enzymatic staining. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
