A rapid method for the purification of D-amino acid oxidase of Trigonopsis variabilis by hydrophobic chromatography |
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| Authors: | Ashwini Deshpande K Sankaran S F D'Souza G B Nadkarni |
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| Institution: | (1) Food Technology & Enzyme Engineering Division, Bhabha Atomic Research Centre, 400 085 Bombay, India |
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| Abstract: | Summary A relatively simple method has been described for the rapid purification of D-amino acid oxidase from Trigonopsis variabilis by hydrophobic chromatography on Phenyl-Sepharose CL-4B and negative adsorption on DEAE-cellulose. The purified enzyme had a specific activity of 22–24 units at 25°C and exhibited three bands on enzymatic staining. |
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