Industrial yeast strain improvement: construction of strains having the killer character and capable of utilizing starch |
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Authors: | Adela Bortol Clara Nudel Ana M Giulietti J F T Spencer D M Spencer |
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Institution: | (1) Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina;(2) Goldsmiths' College, Department of Life Sciences, University of London, Rachel McMillan Building, Creek Road, SE8 3BU London, England |
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Abstract: | Summary A hybrid of Saccharomyces cerevisiae with the ability to utilize starch and to produce the killer toxin was constructed by the protoplast fusion technique. The hybrid was obtained in two steps. In the first, a wild killer strain was fused with a laboratory strain (S. cerevisiae STA2). A fusion product which carried the killer factor and the ability to grow on starch was selected. In the second step, this hybrid was fused with a baker's yeast. |
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