Overexpression of tomato <Emphasis Type="Italic">tAPX</Emphasis> gene in tobacco improves tolerance to high or low temperature stress |
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| Authors: | W -H Sun M Duan F Li D -F Shu S Yang Q -W Meng |
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| Institution: | (1) Department of Horticulture, School of Agriculture and Food Science, Zhejiang A and F University, Lin’an, 311300, Zhejiang, People’s Republic of China;(2) The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, Beijing, 10081, People’s Republic of China; |
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| Abstract: | In order to investigate the function of chloroplast ascorbate peroxidase under temperature stress, the thylakoid-bound ascorbate
peroxidase gene from tomato leaf (TtAPX) was introduced into tobacco. Transformants were selected for their ability to grow on medium containing kanamycin. RNA gel
blot analysis confirmed that TtAPX in tomato was induced by chilling or heat stress. Over-expression of TtAPX in tobacco improved seed germination under temperature stress. Two transgenic tobacco lines showed higher ascorbate peroxidase
activity, accumulated less hydrogen peroxide and malondialdehyde than wild type plants under stress condition. The photochemical
efficiency of photosystem 2 in the transgenic lines was distinctly higher than that of wild type plants under chilling and
heat stresses. Results indicated that the over-expression of TtAPX enhanced tolerance to temperature stress in transgenic tobacco plants. |
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