The microscopic biological response of human chondrocytes to bovine bone scaffold |
| |
Authors: | Baharudin Abdullah Abdul Halim Shibghatullah Suzina Sheikh Abdul Hamid Nor Shamsuria Omar Abd Rani Samsuddin |
| |
Institution: | (1) Universiti Sains Malaysia, Kubang Kerian, Malaysia |
| |
Abstract: | This study was performed to determine the microscopic biological response of human nasal septum chondrocytes and human knee
articular chondrocytes placed on a demineralized bovine bone scaffold. Both chondrocytes were cultured and seeded onto the
bovine bone scaffold with seeding density of 1 × 105 cells per 100 μl/scaffold and incubated for 1, 2, 5 and 7 days. Proliferation
and viability of the cells were measured by mitochondrial dehydrogenase activity (MTT assay), adhesion study was analyzed
by scanning electron microscopy and differentiation study was analyzed by immunofluorescence staining and confocal laser scanning
electron microscopy. The results showed good proliferation and viability of both chondrocytes on the scaffolds from day 1
to day 7. Both chondrocytes increased in number with time and readily grew on the surface and into the open pores of the scaffold.
Immunofluorescence staining demonstrated collagen type II on the scaffolds for both chondrocytes. The results showed good
cells proliferation, attachment and maturity of the chondrocytes on the demineralized bovine bone scaffold. The bovine bone
being easily resourced, relatively inexpensive and non toxic has good potential for use as a three dimensional construct in
cartilage tissue engineering. |
| |
Keywords: | Chondrocytes Bovine bone Scaffold In vitro |
本文献已被 PubMed SpringerLink 等数据库收录! |