Elimination of amino acid losses with protein acid hydrolyzates due to adsorption |
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Authors: | E Robel |
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Affiliation: | Biology Department, Yale University, New Haven, Connecticut 06520 USA |
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Abstract: | A relatively convenient and rapid chromatographic method has been devised for the preparation of gram quantities of the main component of human hemoglobin. The product of this preparative method has been examined for the presence of minor hemoglobin components, ferrihemoglobin, and phosphates. The amounts of each of these materials were found to be small. Measurements of the oxygen-binding isotherm and tetramer-dimer dissociation constant of the product were also carried out, and its behavior was found to be consistent with that observed by other investigators for unfractionated, phosphate-free hemoglobin. |
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