Development of patatin knockdown potato tubers using RNA interference (RNAi) technology,for the production of human-therapeutic glycoproteins |
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Authors: | Yoon-Sik Kim Yong-Hwa Lee Hyun-Soon Kim Mi-Sun Kim Kyu-Woong Hahn Jeong-Heon Ko Hyouk Joung Jae-Heung Jeon |
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Institution: | (1) Plant Genome Research Center, KRIBB, Daejeon, 305-806, Korea;(2) Department of Biological Sciences, Hannam University, Daejeon, 306-791, Korea;(3) Daejeon-KRIBB-FHCRC Research Cooperation Center, KRIBB, Daejeon, 305-806, Korea |
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Abstract: | Background Patatins encoded by a multi-gene family are one of the major storage glycoproteins in potato tubers. Potato tubers have recently
emerged as bioreactors for the production of human therapeutic glycoproteins (vaccines). Increasing the yield of recombinant
proteins, targeting the produced proteins to specific cellular compartments, and diminishing expensive protein purification
steps are important research goals in plant biotechnology. In the present study, potato patatins were eliminated almost completely
via RNA interference (RNAi) technology to develop potato tubers as a more efficient protein expression system. The gene silencing
effect of patatins in the transgenic potato plants was examined at individual isoform levels. |
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