首页 | 本学科首页   官方微博 | 高级检索  
     


Lytic effect ofVibrio cholerae elastase on Gram-positive and-negative bacteria
Authors:M. Kaur  M. Gupta  K. K. Tripathi  K. G. Gupta
Affiliation:(1) Department of Microbiology, Panjab University, 160 014 Chandigarh, India;(2) Central Research Institute, Kasauli, India
Abstract:Elastase ofVibrio cholerae caused the lysis of freshly grown cells of Gram-negative (Pseudomonas aeruginosa, Proteus vulgaris, Salmonella paratyphi A andKlebsiella pneumoniae) bacteria. Gram-positive (Staphylococcus aureus andS. epidermidis) organisms were resistant to this enzyme. Heat killed and lyophilized Gram-positive and-negative bacteria (exceptS. aureus andS. epidermidis) showed higher sensitivity to elastase. Both Gram-negative and-positive bacteria were lyzed maximally by elastase at pH 8.0. At this pH, lytic activity of elastase was maximum in Tris-HCl and glycine-NaOH buffers followed by Tris-maleate and cacodylate buffers.
Keywords:
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号