Lytic effect ofVibrio cholerae elastase on Gram-positive and-negative bacteria |
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Authors: | M. Kaur M. Gupta K. K. Tripathi K. G. Gupta |
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Affiliation: | (1) Department of Microbiology, Panjab University, 160 014 Chandigarh, India;(2) Central Research Institute, Kasauli, India |
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Abstract: | Elastase ofVibrio cholerae caused the lysis of freshly grown cells of Gram-negative (Pseudomonas aeruginosa, Proteus vulgaris, Salmonella paratyphi A andKlebsiella pneumoniae) bacteria. Gram-positive (Staphylococcus aureus andS. epidermidis) organisms were resistant to this enzyme. Heat killed and lyophilized Gram-positive and-negative bacteria (exceptS. aureus andS. epidermidis) showed higher sensitivity to elastase. Both Gram-negative and-positive bacteria were lyzed maximally by elastase at pH 8.0. At this pH, lytic activity of elastase was maximum in Tris-HCl and glycine-NaOH buffers followed by Tris-maleate and cacodylate buffers. |
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