发展中的DNA测序技术

发展中的ＤＮＡ测序技术赵晓娟刘金毅综述蔡有余琦祖和＊审校（中国医学科学院中国协和医科大学实验动物研究所北京）ＤＮＡ序列分析是基因工程和分子生物学领域最重要的技术之一，是了解基因结构和功能的基础。“人类基因组计划”（ｈｕｍａｎｇｅｎｏｍｅｐｒｏｊｅｃｔ）的实施，有力地推动了高速ＤＮＡ测序技术的发展。除经典的测序方法在技术环节上的不断改进外，近年来发展了一些全新的ＤＮＡ测序方法，如毛细管凝胶电泳...

Developing DNA Sequencing Technique

DNA sequence analysis is one of the most important techniques in gene engineering and molecular biology, and basis of studying the structrue and fuction of genes. Human genome project compel us to develop high speed DNA sequencing. Except that classical methods have been improved in detail, a few new DNA sequencing methods arise, such as capillary array electrophoresis , ultrathin slab gel electrophoresis, sequencing by hybridization, mass spectrometry, atomic probe microscopy and single molecule fluorescence detection in flowing sample streams.