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Specific Oligoribonucleotide Synthesis Using Primer-Independent Polynucleotide Phosphorylase |
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| Authors: | Robert G. Haváer David B. Straus |
| Affiliation: | 1. Department of Biochemistry , State University of New York , Buffalo, New York, 14214;2. Long Island Jewish-Hillside Medical Center , New Hyde Park, N. Y., 11040;3. Dept. of Chemistry , State University of New York , New Paltz, New York, 12561 |
| Abstract: | Oligoribonucleotides of a predetermined base sequence begin-ing with adenylyl-3′ 5′-adenosine at the 5′ end have been synthesized in yields varying between 13% and 42%. The synthesis was carried out using primer-independent polynucleotide phosphory-lase from E. coli in the presence of high concentrations of primer and of sodium chloride. |
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