Purification of Canine Plasma Renin by Affinity Chromatography |
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| Authors: | Robert Walsh Dean T. Mason Melvln J. Tonkon Joan Wikman-coffelt |
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| Affiliation: | Section of Cardiovascular Medicine, Department of Medicine , University of California, Davis School of Medicine |
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| Abstract: | An affinity column for the purification of canine plasma renin was prepared using goat anti-renin (dog kidney) γG globulins. The antiserum was prepared against a purified kidney renin preparation. The anti-renin globulins were coupled to cyanogen bromide-activated Sepharose. Using the anti-renin globulin-coupled Sepha-rose as an immuno-adsorbant, a method was devised allowing purification of plasma renin to a 1, 000-fold purity. |
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