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Purification of L-Isoleucyl t-rna Synthetase by Affinity Chromatography |
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| Authors: | Petrie Rainey Edward L. Bennett Melvin Calvin |
| Affiliation: | Laboratory of Chemical Biodynamics, Lawrence Berkeley Laboratory and the Department of Chemistry , University of California , Berkeley, Calif., 94720 |
| Abstract: | L-Isoleucyl t-RNA synthetase was purified to 97% purity in a single step by affinity chromatography on a column carrying L-isoleucinyl 5′-adenylate as the insolubilized ligand. Recovery was 50–60%. |
| Keywords: | Chimeric synthetic peptides UMELISA HIV‐1 gp41 gp120 |