Detection of an antigenic cell wall layer inHistoplasma capsulatum |
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Authors: | Mercedes R Edwards Katherine E Fritz |
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Institution: | (1) Wadsworth Center for Laboratories and Research, New York State Department of Health, 12201 Albany, NY;(2) VA Medical Center and Albany Medical College, 12208 Albany, NY, USA |
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Abstract: | Histoplasma capsulatum yeast cells have been studied by immunoelectron microscopy using rabbit polyclonal antisera and a biotin-avidin-peroxidase detection system. An antigenic surface layer has been visualized in the cell wall of immunostained organisms. This layer was not seen in samples prepared by standard electron microscopic methods or in negative controls used with the immunocytochemical technique. Without immunostaining the cell wall ofHistoplasma appeared almost transparent. In contrast, after immunoperoxidase staining the cell wall was conspicuous, bounded by the darkly stained outer layer. This electron dense layer, appeared to be a reservoir of surface antigens that were recognized by anti-Histoplasma antibodies.Abbreviations CHHA
Cystine-heart-hemoglobin agar
- PBS
phosphate buffered saline
- Ig
immunoglobulin
- TBS
Tris buffered saline
- DAB
3,3-diaminobenzidine tetrachloride
- FITC
fluorescein isothiocyanate
- M199
tissue culture medium 199, according to Morgan et al. (1950) |
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Keywords: | Histoplasma capsulatum Surface antigens Immunoelectron microscopy Biotin-avidin-peroxidase |
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