自身抗体引起的ABO血型正反定型结果不一致

目的:对因自身抗体引起ABO血型正反定型结果不一致的疑难血标本进行血型鉴定和分析,探讨其原因和解决办法。方法:先用直接抗人球蛋白试验(DAT)和间接抗人球蛋白试验(IAT)确定ABO血型正反定型结果不一致的原因是由于自身抗体的存在,然后做吸收放散试验、抗体筛选试验等排除自身抗体的干扰以便血型的正确判定。结果:ABO血型正反定型结果不一致因自身抗体引起的有10例,其中温抗体7例,冷抗体3例,温抗体同时同种抗体阳性2例,冷抗体同时同种抗体阳性2例。结论:自身抗体会影响ABO血型的正确判定,选用合适的试验进行分析判断以提高血型鉴定的准确性。

Positive and Negative Stereotypes Inconsistent in ABO Blood Group Caused by Autoantibody

Objective:To analyze the difficult blood samples whose positive and negative stereotypes inconsistent in ABO blood group caused by autoantibody, and to explore the reasons and solutions.Methods:First, the reason of positive and negative stereotypes inconsistent in ABO blood group was found to be the antibodies with DAT and IAT. Then absorption-elution test and antibody screening test was done to eliminate the interference of autoantibodies in order to determine blood type correctly.Results:Antibodies caused positive and negative stereotypes inconsistent in ABO blood group in 1 0 cases: warm antibody type in 7 cases, cold antibody type in 3 cases, warm antibody combine irregular antibody in 2 cases, and cold antibody combine allo-antibody in 2 cases.Conclusion:Autoantibody can affect accurate judgment of ABO blood group, and appropriate tests should be chosen to improve the accuracy of the blood typing.