湖北双蝴蝶的组织培养研究

以湖北双蝴蝶带芽茎段、不带芽茎段及叶片为外植体,以MS为基本培养基,通过添加不同的植物生长物质种类和浓度配比,建立湖北双蝴蝶组培快繁体系。结果如下:在所有实验方案中,带芽茎段的出愈率最高,是理想的离体快繁材料。较适宜的初代培养基为MS+BA2.0mg/L+蔗糖3.0%,增殖培养基为MS+BA2.0mg/L+NAA0.1mg/L+蔗糖3.0%,而根的诱导则在1/2MS+NAA0.5mg/L+蔗糖1.5%的培养基上进行较为适宜。同时对组织培养过程中湖北双蝴蝶植株再生的方式进行了讨论。

Tissue culture of Tripterospermum discoideum

The tissue culture and rapid proliferation techniques of Tripterospermum discoideum were studied by using stems with buds,stems without buds and leaves as explants.The explants were cultivated in different MS media with different types and concentrations of plant growth regulators.The main results can be concluded as follows:the stems with buds were the best material in speeding propagation among the three explants.The shoot differentiation was MS+BA2.0 mg/L+saccharose3.0%,the optimum medium for proliferation was MS+BA2.0 mg/L+NAA0.1 mg/L+saccharose3.0%,and best medium for rooting was 1/2MS+NAA0.5 mg/L+saccharose1.5%.At the same time,the mode of plant regeneration of T.discoideum in tissue culture has been discussed.