Detection of Homoserine Lactone-Like Quorum Sensing Molecules in Bradyrhizobium Strains |
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| Authors: | Neelawan Pongsilp Eric W. Triplett Michael J. Sadowsky |
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| Affiliation: | (1) Department of Agronomy, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA;(2) Department of Soil, Water, and Climate, and BioTechnology Institute, University of Minnesota, St. Paul, Minnesota 55108, USA;(3) Present address: Department of Microbiology, University of SanamChandra Palace Campus, Nakhon Pathom, 73000, Thailand;(4) Present address: Department of Microbiology and Cell Science, University of Florida, Gainesville, FL 32611-0700, USA |
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| Abstract: | ![]()
One hundred and forty-two Bradyrhizobium strains were screened for their ability to produce N-acyl homoserine lactone-like molecules (AHLs) by using an Agrobacterium tumefaciens biosensor strain containing a traI-lacZ fusion. Approximately 22% (31 of 142) of the tested strains produced AHLs that induced moderate to elevated β-galactosidase activity levels in the biosensor strain. Bradyrhizobium japonicum and Bradyrhizobium elkanii strains were both shown to produce AHLs. Age of culture, and media composition were each shown to influence production of AHL(s), with greater production occurring in 2 day-old cultures grown in rich media. Reverse-phase high-performance liquid chromatography and thin-layer chromatography analyses indicated that the B. japonicum strain USDA 290 produced at least two types of AHLs. Our results indicate that the production AHL-like autoinducers is widespread among both B. japonicum and B. elkanii strains. |
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