Novel cellulases from an extremophilic filamentous fungi <Emphasis Type="Italic">Penicillium citrinum</Emphasis>: production and characterization |
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Authors: | Tanmay Dutta Rupam Sahoo Rajib Sengupta Sougata Sinha Ray Arindam Bhattacharjee Sanjay Ghosh |
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Institution: | (1) Department of Biochemistry, University College of Science, Calcutta University, 35, Ballygunge Circular Road, Kolkata, 700 019, West Bengal, India |
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Abstract: | The enzymatic hydrolysis of cellulose has potential economical and environment-friendly applications. Therefore, discovery
of new extremophilic cellulases is essential to meet the requirements of industry. Penicillium citrinum (MTCC 6489) that was previously isolated from soil in our laboratory, produced alkali tolerant and thermostable cellulases.
Endoglucanase and filter paper activity hydrolase (FPAse) production of P. citrinum were studied using wheat bran substrate in solid state and submerged culture. Zymogram analysis of endoglucanase revealed
the presence of two isoforms differing in molecular weight. One of them was 90 kDa and other one was 38 kDa. Partially purified
endoglucanase showed two different peaks at pH 5.5 and 8.0, respectively, in its pH optima curve. But FPase showed only one
peak (at pH 6.5) in its pH optima curve. Cellulase of P. citrinum is thermostable in nature. The present work reports for the first time, the alkali stable cellulase from alkali tolerant
fungus Penicillium citrinum. Thermostable endoglucanase from P. citrinum may have potential effectiveness as additives to laundry detergents. |
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Keywords: | Alkali tolerant fungus Extremophilic cellulase Penicillium citrinum Solid-state fermentation Wheat bran matrix |
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